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In vitro experiments, 25C-NBOMe has been proven to exhibit cytotoxicity on neuronal cell strains SH-SY5Y, PC12, and SN471, as well as the compound was far more strong than methamphetamine at minimizing the visibility with the respective cells; the neurotoxicity from the compound entails activation of MAPK/ERK cascade and inhibition of Akt/PKB signa